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human liposarcoma cell line sw872 (Procell Inc)

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Procell Inc human liposarcoma cell line sw872
Human Liposarcoma Cell Line Sw872, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+liposarcoma+cell+line+sw872/human+liposarcoma+cell+line+sw872/pmc11467964-74-1-15
Average 90 stars, based on 1 article reviews

human liposarcoma cell line sw872 - by Bioz Stars, 2026-09

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Article Title: Comprehensive analysis of prognostic and immunological role of basement membrane‐related genes in soft tissue sarcoma
Article Snippet: The human liposarcoma cell line (SW872) and synovial sarcoma cell line (SYO‐1) were acquired from Procell Life Science & Technology Co., Ltd. (Hubei, China).

Article Title: A Necroptosis-Related lncRNA Signature Predicts Prognosis and Indicates the Immune Microenvironment in Soft Tissue Sarcomas
Article Snippet: The human liposarcoma cell line (SW872) was obtained from Procell Life Science&Technology Co., Ltd. (Hubei, China).

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IL-4-Induced M2 macrophage polarization promotes the progression of LPS. RAW264.7 cells were divided into two groups: control group, IL-4 group. (A) Flow cytometry was used to detect the expression level of CD206 on RAW264.7 cells in each group. (B) ELISA was performed to measure the secretion level of IL-10 in RAW264.7 cells from each group. (C) Western Blot was employed to determine the protein expression level of Arg-1 in RAW264.7 cells of each group. A Transwell co-culture system was utilized, which included two groups: control co-culture group (LPS cells co-cultured with untreated RAW264.7 cells). IL-4 co-culture group (LPS cells co-cultured with RAW264.7 cells pre-treated with IL-4). (D) EdU incorporation assay was used to detect the proliferative activity of SW872 cells and 94T778 cells in each group. (E-F) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 cells and 94T778 cells in each group. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: IL-4-Induced M2 macrophage polarization promotes the progression of LPS. RAW264.7 cells were divided into two groups: control group, IL-4 group. (A) Flow cytometry was used to detect the expression level of CD206 on RAW264.7 cells in each group. (B) ELISA was performed to measure the secretion level of IL-10 in RAW264.7 cells from each group. (C) Western Blot was employed to determine the protein expression level of Arg-1 in RAW264.7 cells of each group. A Transwell co-culture system was utilized, which included two groups: control co-culture group (LPS cells co-cultured with untreated RAW264.7 cells). IL-4 co-culture group (LPS cells co-cultured with RAW264.7 cells pre-treated with IL-4). (D) EdU incorporation assay was used to detect the proliferative activity of SW872 cells and 94T778 cells in each group. (E-F) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 cells and 94T778 cells in each group. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Culture Assay, Cell Culture, Activity Assay, Migration

mTORC2 regulates TAM via PPAR-γ/CD36 pathway to promote LPS progression. LPS cells (SW872 and 94T778) were co-cultured with RAW264.7 macrophages, and the groups were divided as follows: control co-culture group, IL-4 co-culture group, IL-4+JR-AB2-011 co-culture group, IL-4+JR-AB2-011+LPA co-culture group. (A) EdU assay was used to detect the proliferation of SW872 and 94T778 cells in each co-culture group. (B-C) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 and 94T778 cells in each co-culture group. Data are presented as the mean±SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: mTORC2 regulates TAM via PPAR-γ/CD36 pathway to promote LPS progression. LPS cells (SW872 and 94T778) were co-cultured with RAW264.7 macrophages, and the groups were divided as follows: control co-culture group, IL-4 co-culture group, IL-4+JR-AB2-011 co-culture group, IL-4+JR-AB2-011+LPA co-culture group. (A) EdU assay was used to detect the proliferation of SW872 and 94T778 cells in each co-culture group. (B-C) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 and 94T778 cells in each co-culture group. Data are presented as the mean±SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Culture, Control, Co-Culture Assay, EdU Assay, Migration

Validation of the function of TNFSF14 by in vitro experiments. (A) TNFSF14 overexpressed was confirmed by western blot assays in SW872 and HT1080 cells. (B, C) CCK-8 assays showed reduced cell proliferation in TNFSF14 overexpressed cells. (D–G) Cell migration and invasion of sarcoma cells were determined by Transwell assays. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns, no significance.

Journal: Frontiers in Immunology

Article Title: Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma

doi: 10.3389/fimmu.2024.1372692

Figure Lengend Snippet: Validation of the function of TNFSF14 by in vitro experiments. (A) TNFSF14 overexpressed was confirmed by western blot assays in SW872 and HT1080 cells. (B, C) CCK-8 assays showed reduced cell proliferation in TNFSF14 overexpressed cells. (D–G) Cell migration and invasion of sarcoma cells were determined by Transwell assays. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns, no significance.

Article Snippet: In this experiment, the human liposarcoma cell line SW872 (CL-0685B) and the human fibrosarcoma cell line HT1080 (CL-0117) were purchased from Procell Biotech (Wuhan, China), and passed STR identification and mycoplasma contamination testing.

Techniques: Biomarker Discovery, In Vitro, Western Blot, CCK-8 Assay, Migration

Figure 1. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce cell senescence in HepG2 hepatocytes: (a) Representative images of SA-β-GAL staining of HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal (scale bar = 100 µm); (b) Quantiﬁcation of SA-β-GAL positive cells normalized by total cells in the ﬁeld; and (c) SA-β-GAL absorbance recorded at 630 nm. Each dot represents an individual experiment (n = 6). * p < 0.05 versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

Article Title: Secretory Factors from Calcium-Sensing Receptor-Activated SW872 Pre-Adipocytes Induce Cellular Senescence and A Mitochondrial Fragmentation-Mediated Inflammatory Response in HepG2 Cells.

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 1. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce cell senescence in HepG2 hepatocytes: (a) Representative images of SA-β-GAL staining of HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal (scale bar = 100 µm); (b) Quantiﬁcation of SA-β-GAL positive cells normalized by total cells in the ﬁeld; and (c) SA-β-GAL absorbance recorded at 630 nm. Each dot represents an individual experiment (n = 6). * p < 0.05 versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Staining, Control

Figure 2. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that activate the p16/p21/p53 cascade in HepG2 cells: (a) immunodetection of p53, p21, and p16 protein levels in HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal; (b) quantiﬁcation of p53 protein levels; (c) quantiﬁcation of p21 protein levels; (d) quantiﬁcation of p16 protein levels. Each dot represents an individual experiment (n = 5–6). * p < 0.05, versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 2. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that activate the p16/p21/p53 cascade in HepG2 cells: (a) immunodetection of p53, p21, and p16 protein levels in HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal; (b) quantiﬁcation of p53 protein levels; (c) quantiﬁcation of p21 protein levels; (d) quantiﬁcation of p16 protein levels. Each dot represents an individual experiment (n = 5–6). * p < 0.05, versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Immunodetection, Control

Figure 3. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce cell cycle arrest in HepG2 cells: (a) representative images of HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal. Hoechst (blue) was used to identify the nuclei and anti-Ki67 antibody (green) to evaluate the nuclear localization of Ki67 (scale bar = 100 µm); (b) quantiﬁcation of total Ki67 immunoﬂuorescence. The inset shows CMcal immunoﬂuorescence relative to the control. Each dot represents an individual experiment (n = 5). * p < 0.05 versus the control, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 3. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce cell cycle arrest in HepG2 cells: (a) representative images of HepG2 cells exposed to the CMveh, CMcin, and CMcin+cal. Hoechst (blue) was used to identify the nuclei and anti-Ki67 antibody (green) to evaluate the nuclear localization of Ki67 (scale bar = 100 µm); (b) quantiﬁcation of total Ki67 immunoﬂuorescence. The inset shows CMcal immunoﬂuorescence relative to the control. Each dot represents an individual experiment (n = 5). * p < 0.05 versus the control, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Control

Figure 4. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce the secretion of pro-inﬂammatory cytokines in HepG2 cells: relative abundance of (a) IL-1β and (b) CCL2 mRNA in HepG2 cells exposed to CMcin and CMcin+cal; expressed as fold from CMveh, (represented by the dotted line). mRNA levels were normalized to GAPDH mRNA. The insets show CMcal normalized mRNA levels relative to the control. Each dot represents an individual experiment (n = 2–7). * p < 0.05 versus the control, # p < 0.05 versus CMcin, and the Wilcoxon signed rank test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 4. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce the secretion of pro-inﬂammatory cytokines in HepG2 cells: relative abundance of (a) IL-1β and (b) CCL2 mRNA in HepG2 cells exposed to CMcin and CMcin+cal; expressed as fold from CMveh, (represented by the dotted line). mRNA levels were normalized to GAPDH mRNA. The insets show CMcal normalized mRNA levels relative to the control. Each dot represents an individual experiment (n = 2–7). * p < 0.05 versus the control, # p < 0.05 versus CMcin, and the Wilcoxon signed rank test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Control

Figure 5. CaSR activation in SW872 pre-adipocytes elevates IL-1β and TNF-α secretion, which induce cell senescence in HepG2 cells: (a) immunodetection of IL-6, IL-1β, and TNF-α protein levels in CMcin after immunoprecipitation (n = 1); (b) immunodetection of IL-6, IL-1β, and TNF-α protein levels in the CMcin pellet after immunoprecipitation (n = 1); (c) Representative images of SA-β-GAL staining of HepG2 cells exposed to CMcin, CMIL-6 [−], CMIL-1β [−], and CMTNF-α [−] (scale bar = 100 µm); (d) quantiﬁcation of SA-β-GAL-positive cells normalized by total cells in the ﬁeld; (e) SA-β-GAL absorbance recorded at 630 nm. Each dot represents an individual experiment (n = 6). * p < 0.05, and ** p < 0.01, the versus control, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 5. CaSR activation in SW872 pre-adipocytes elevates IL-1β and TNF-α secretion, which induce cell senescence in HepG2 cells: (a) immunodetection of IL-6, IL-1β, and TNF-α protein levels in CMcin after immunoprecipitation (n = 1); (b) immunodetection of IL-6, IL-1β, and TNF-α protein levels in the CMcin pellet after immunoprecipitation (n = 1); (c) Representative images of SA-β-GAL staining of HepG2 cells exposed to CMcin, CMIL-6 [−], CMIL-1β [−], and CMTNF-α [−] (scale bar = 100 µm); (d) quantiﬁcation of SA-β-GAL-positive cells normalized by total cells in the ﬁeld; (e) SA-β-GAL absorbance recorded at 630 nm. Each dot represents an individual experiment (n = 6). * p < 0.05, and ** p < 0.01, the versus control, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Immunodetection, Immunoprecipitation, Staining, Control

Figure 6. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that alter proteins governing mitochondrial dynamics in HepG2 cells: (a) immunodetection of OPA1 and MFN2 protein levels in HepG2 cells exposed to CMveh, CMcin, and CMcin+cal; (b) immunodetection of PGC-1α and DRP1 protein levels in treated HepG2 cells; quantiﬁcation of (c) OPA1; (d) MFN2; (e) PGC-1α; and (f) DRP1 protein levels. Each dot represents an individual experiment (n = 4–5). * p < 0.05 and ** p < 0.01 versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 6. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that alter proteins governing mitochondrial dynamics in HepG2 cells: (a) immunodetection of OPA1 and MFN2 protein levels in HepG2 cells exposed to CMveh, CMcin, and CMcin+cal; (b) immunodetection of PGC-1α and DRP1 protein levels in treated HepG2 cells; quantiﬁcation of (c) OPA1; (d) MFN2; (e) PGC-1α; and (f) DRP1 protein levels. Each dot represents an individual experiment (n = 4–5). * p < 0.05 and ** p < 0.01 versus the control, # p < 0.05 versus CMcin, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Immunodetection, Control

Figure 7. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce mito- chondrial alterations in HepG2 cells: (a) representative immunoﬂuorescence images obtained through confocal microscopy of HepG2 cells treated with CMveh, CMcin, or CMcin+cal stained with MitoTracker Orange (MTO, red) and an anti-mtHsp70 antibody (green); the ratio of MTO/mtHSP70 ﬂuorescence is shown in pseudo colors (right column of images); (b) quantiﬁcation of the overall ﬂuorescence ratios between MTO mtHsp70; (c) quantiﬁcation of the mean mitochondrial area; (d) quantiﬁcation of the average number of mitochondria per cell. The insets show CMcal levels relative to the control. Each dot represents an individual experiment (n = 4). * p < 0.05 and ** p < 0.01 versus the control, Friedman’s test, and Dunn’s post-hoc test.

Journal: International journal of molecular sciences

doi: 10.3390/ijms24065217

Figure Lengend Snippet: Figure 7. CaSR activation in SW872 pre-adipocytes induces the secretion of factors that induce mito- chondrial alterations in HepG2 cells: (a) representative immunoﬂuorescence images obtained through confocal microscopy of HepG2 cells treated with CMveh, CMcin, or CMcin+cal stained with MitoTracker Orange (MTO, red) and an anti-mtHsp70 antibody (green); the ratio of MTO/mtHSP70 ﬂuorescence is shown in pseudo colors (right column of images); (b) quantiﬁcation of the overall ﬂuorescence ratios between MTO mtHsp70; (c) quantiﬁcation of the mean mitochondrial area; (d) quantiﬁcation of the average number of mitochondria per cell. The insets show CMcal levels relative to the control. Each dot represents an individual experiment (n = 4). * p < 0.05 and ** p < 0.01 versus the control, Friedman’s test, and Dunn’s post-hoc test.

Article Snippet: A human liposarcoma-derived SW872 pre-adipose cell line (HTB-92, ATCC, Manassas, VA, USA) was grown in DMEM/F12 medium.

Techniques: Activation Assay, Confocal Microscopy, Staining, Control

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human liposarcoma cell line sw872 (Procell Inc)

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